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2 edition of Methods for determining bactericidal activity of antimicrobial agents found in the catalog.

Methods for determining bactericidal activity of antimicrobial agents

National Committee for Clinical Laboratory Standards.

Methods for determining bactericidal activity of antimicrobial agents

by National Committee for Clinical Laboratory Standards.

  • 352 Want to read
  • 23 Currently reading

Published by National Committee for Clinical Laboratory Standards in Villanova, PA .
Written in English

    Subjects:
  • Anti-infective agents -- Testing -- Standards.

  • Edition Notes

    Statementprocedures for determining the lethal activity of antimicrobial agents.
    SeriesNCCLS document ;, M26-P = vol. 7, no. 2, NCCLS document ;, vol. 7, no. 2.
    Classifications
    LC ClassificationsQR69.A56 N38 1987
    The Physical Object
    Paginationvii, p. 35-76 ;
    Number of Pages76
    ID Numbers
    Open LibraryOL2483898M
    LC Control Number87404456

    A method for determining simultaneous bactericidal and bacteriostatic activity of an antimicrobial agent by measuring a time rate of change, expressed as A, of a function f(N) having a number of viable cells, expressed as N, in a microbial population with time of exposure to a selected concentration of an antimicrobial agent with the function f. Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline Abstract Established laboratory methods that can assess the bactericidal activity of an antimicrobial agent are needed, both because of the increase in the number of patients who do not have completely normal host immune defenses and because of the new.

    The authors provide a comprehensive, up-to-date procedural manual including protocols for bioassay methods and molecular methods for bacterial strain typing. Divided into three sections, the text begins by introducing basic susceptibility disciplines including disk diffusion, macro and micro broth dilution, agar dilution, and the gradient method. IBRG TEX13// –Quantitative Method for Evaluation Bactericidal Activity of Textiles and Porous Materials and Articles OECD –Guidance Document for Quantitative Method for Evaluating Antibacterial Activity of Porous and Non-porous Antibacterial Treated Articles (ENV/JM/MOMO()18) International Antimicrobial Council ().

    The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated. MIC values are used to determine suscep . CLSI also has developed methods specific for the yeasts, filamentous fungi, and anaerobic bacteria. For MBC determination, CLSI MA, Methods for Determining Bactericidal Activity of Antimicrobial Agents, is an accepted industry standard.


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Methods for determining bactericidal activity of antimicrobial agents by National Committee for Clinical Laboratory Standards. Download PDF EPUB FB2

In order to determine the relationship between two antimicrobial agents, an additional method such as the checkerboard assay and the E test method.

Methods for determining bactericidal activity of antimicrobial agents by National Committee for Clinical Laborato Published by National Committee for Clinical Laboratory Standards in Villanova, PA.

Written in EnglishPages: Get this from a library. Methods for determining bactericidal activity of antimicrobial agents: approved guideline. [Arthur L Barry; National Committee for Clinical Laboratory Standards.].

Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline This document provides procedures for determining the lethal activity of antimicrobial agents.

A guideline for global application developed through the Clinical and Laboratory Standards Institute consensus Size: 1MB. methods for determining bactericidal activity of antimicrobial agents by National Committee for Clinical Laboratory Standards.; 2 editions; First published in ; Subjects: Anti-infective agents, Standards, Testing.

Buy Methods for Determining Bactericidal Activity of Antimicrobial Agents: Approved Guideline (NCCLS document) by Arthur L. Barry (ISBN: ) from Amazon's Book Store. Everyday low prices and free delivery on eligible : Arthur L. Barry. bacterial resistance to traditionally used antimicrobials, it has become more difficult for clinicians to empirically select an appropriate antimicrobial agent.

As a result, in-vitro antimicrobial susceptibility testing (AST) of the relevant bacterial pathogens, from properly collected specimens, should use validated methods.

Time-kill test is the most appropriate method for determining the bactericidal or fungicidal effect. It is a strong tool for obtaining information about the dynamic interaction between the antimicrobial agent and the microbial strain.

The time-kill test reveals a time-dependent or a concentration-dependent antimicrobial effect. As common microbiological methods for the assessment of bacteriostatic or bactericidal activities are very time-consuming, in this work we describe that the use of a novel far-red fluorescent.

Single Tube Method for Determining the Efficacy of Disinfectants against Bacterial Biofilm: MB Quantitative Three Step Method for Measuring the Efficacy of Liquid Sporicides against Spores of Bacillus subtilis on Hard Non-porous and Porous Surfaces: MB Preparation and Sampling Procedures for Antimicrobial Test Substances: MB Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline This document provides procedures for determining the lethal activity of antimicrobial agents.

A guideline for global application developed through the Clinical and Laboratory Standards Institute consensus process. September Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline Foreword All of the susceptibility test methods commonly performed by clinical microbiology laboratories (e.g., disk diffusion, broth dilution, and agar dilution) measure the inhibitory activity.

CLSI MA Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Established laboratory methods that can assess the bactericidal activity of an antimicrobial agent are needed, both because of the increase in the number of patients who do not have completely normal host immune defenses and because of the new classes of.

The emergence of resistance to antimicrobial agents is a pressing concern for human health that increases the need for the development of novel antimicrobial drugs.

Antimicrobial resistance means that microorganism keep on growing even in the presence of a drug due to specific defense mechanisms [e.g. efflux-pumps]. This method can confirm the presence of antimicrobial activity in plastics or hydrophobic surfaces and allows determination of quantitative differences in antimicrobial activity between untreated plastics or polymers and those with bound or incorporated low water-soluble antimicrobial agents.

Comparisons between the numbers of survivors on preservative. Buy MA, “Methods for Determining Bactericidal Activity of Antimicrobial Agents,” from CLSI. Discover additional Microbiology standards in the CLSI Shop. Methods for Determining Bactericidal Activity and Antimicrobial Interactions: Synergy Testing, Time-Kill Curves, and Population Analysis View abstract chapter 13 | 14 pages.

Some organisms have predictable susceptibility to antimicrobial agents (ie, Streptococcus pyogenes to penicillin), and empirical therapy for these organisms is typically used. Therefore, AST for such pathogens is seldom required or performed.

In addition, AST is valuable in evaluating the activity of new and experimental compounds. Leaching of an antimicrobial is dependent upon the test conditions being utilized and the ultimate end use of the product.

For example, water soluble antimicrobials will be prone to removal from the test surface using the method described in Section 13 but insoluble compounds will not. It is for this reason that the use of the term leaching throughout this document is.

Antimicrobial packaging is a fast emerging area in food packaging. Antimicrobial agents can be incorporated into polymeric food packaging materials to enhance the shelf-life of packaged foods, by preserving the foods against microbial spoilage and hazardous food-borne microorganisms.

This chapter reviews different classes of antimicrobials. The minimum bactericidal concentration (MBC) test can be used to assess the ability of an antimicrobial agent to kill a bacterial isolate.

MBC tests are performed after consultation in very select clinical situations when it is necessary to determine the bactericidal activity of an antimicrobial agent against a bacterial isolate.The minimum bactericidal concentration (MBC) test can be used to assess the ability of an antimicrobial agent to kill a bacterial isolate.

MBC tests are performed after consultation in very select clinical situations when it is necessary to deter-mine the bactericidal activity of an anti-microbial agent against a bacterial isolate.disk-diffusion method 1.

a Petri plate containing an agar growth medium is inoculated uniformly over its entire surface 2. paper disks impregnated with antimicrobial agents are place on surface of agar 3. during incubation, antimicrobial agent diffuses from the disc, from an area of high concentration to an area of low concentration.